Digital LSR 2000 manuales

Manuales del propietario y guías del usuario para Multifuncionales Digital LSR 2000.
Ofrecemos 3 manuales en pdf Digital LSR 2000 para descargar gratis por tipos de documentos: Manual de servicio, Manual de usuario, Guía de usuario


Digital LSR 2000 Manual de usuario (111 paginas)


Marca: Digital | Categoria: Multifuncionales | Tamaño: 4.54 MB |

 

Tabla de contenidos

Droplet Digital

1

Applications Guide

1

Table of Contents

2

Table of Contents

4

1 Droplet Digital

5

QX100/QX200 Workflow

6

PCR Amplification

8

Experimental Considerations

11

8

12

2 Designing Droplet

15

Designing Probes

16

Designing an Assay

17

14

18

Designing Droplet Digital

18

PCR Experiments

18

Sample Preparation

19

16

20

Droplet Generation

21

Software

23

Droplet Reading

24

Data Analysis

24

Merging Wells

26

EvaGreen and Gene Expression

30

Reference

31

3 Absolute Quantification

32

Statistics of ddPCR

34

Copies per Microliter

35

Copies per Droplet

35

Low Concentration Example

36

High Concentration Example

37

Definitions

38

Errors in ddPCR

40

4 Copy Number

41

38

42

CNV Calculations

43

Assay Design

44

Running a CNV Assay

45

Restriction Digestion

45

42

46

BSA, bovine serum albumin

47

5 Rare Mutation and

49

46

50

Rare Mutation Detection

51

Testing an RMD Assay

52

RMD Experiment Considerations

52

Recommended Controls

56

Rare Sequence Detection

57

RSD Experimental Strategies

58

References

60

6 Gene Expression

61

Obtain RNA

62

Generate cDNA

62

Data Analysis Results

64

Gene Expression

65

7 Next-Generation

68

Decreasing size of library

70

Adapter dimers

70

Library Balancing

72

1 2 3 4 5 6 7 8 9 10 11 12

73

70

74

Chloroform Aqueous

74

8 Additional Applications

76

Milepost Assay

77

74

78

Additional Applications

78

Multiplexing

79

9 Droplet Digital

82

No or Few Positive Droplets

87

No or Low Total Droplet Count

87

Insufficient Mixing

88

86

90

Target Accessibility

91

88

92

– + – + – +

94

Appendix A

95

Ordering Information

95

92

96

Appendix B

98

Technical Error Bars

98

Partitioning

100

Appendix C

102

Acronyms

102

Appendix C: Acronyms

103

Index

104

102

106

PCR Applications Guide

106

104

108

106

110

Life Science

111

Bio-Rad

111

Laboratories, Inc

111





Más productos y manuales para Multifuncionales Digital

Modelos Tipo de documento
LA400 Manual de usuario   Printer Standards [en] [ja] , 7 paginas
LA400 El manual del propietario   Digital LA400 Owner`s manual [en] , 48 paginas
LA400 El manual del propietario   Digital LA400 Owner`s manual, 32 paginas
LA400 Manual de servicio   Digital LA400 Service manual [en] [fr] , 127 paginas